FAQ: Black Polycarbonate Membranes

When counting bacteria as part of epifluorescent microscopy we generally recommend using the black polycarbonate membranes instead of cellulose membranes. This is because the black polycarbonate materials have a uniform pore size and flat surface that will retain all of the bacteria without trapping any inside of the filter. Though cellulose membranes will retain bacteria, it often will become trapped inside of the filter, where it cannot be counted.