If the goal of your vacuum filtration is purification using a membrane with an absolute pore size, Sterlitech recommends utilizing glass filter holder assemblies. Compared to Buchner funnels, these systems create a tight seal to prevent liquid bypass around the membrane. Without this seal, the benefit of a highly retentive membrane disappears.
It is typical to experience some leaking in many vacuum filtration applications, but minimizing this will significantly improve yield. There are a few ways to ensure optimal sealing, including proper set-up of the apparatus and running under the manufacturer’s recommended conditions.
To illustrate this, we ran trial liquid filtration on our 150 mm assembly with improper gasket placement (below support)