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Hydrophilic PVDF Membranes

These hydrophilic, extremely low extractable/protein binding, PVDF membrane filters provide high flow rates and throughputs for aggressive, solvent-based mobile phase applications, biological sterilization/clarification, and HPLC/analytical sample preparation. 

PVDF membranes do not require the use of wetting agents, transmitting negligible extractables and increasing sample purity during sterilization or clarification procedures. Because of their exceptionally low protein binding levels--even lower than similar nylon, nitrocellulose, and PTFE membranes--our PVDF filters are well-utilized in the preparation of proteinacious solutions for chromatography and other instrumental analyses. Broad chemical compatibility allows these PVDF filters to accommodate a wide range of applications--especially those requiring high flow rates/throughput--including aggressive/non-aggressive acids, alcohols, and solvents in mobile phase. Our versatile PVDF membrane filters are available with pore sizes of either 0.22 or 0.45 μm; however, the 0.22 μm pore size is preferred for the removal of uHPLC contaminants most likely plug columns and damage analytical results.

Start with a custom PVDF membrane sample pack here.

We provide the filters and technical support to help you take your breakthrough product from an idea to a prototype, to commercial scale.

SKUProduct Name Pore Size Diameter (mm) Pack Size Est Ship Price
1370020 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 300 x 300mm, 5/Pk 0.45 300x300 5 1-2 days
$442.37
1370010 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 47mm, 100/Pk 0.2 47 100 1-2 days
$181.43
1370009 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 25mm, 100/Pk 0.2 25 100 1-2 days
$138.67
1370008 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 13mm, 100/Pk 0.2 13 100 1-2 days
$114.41
1370007 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 90mm, 50/Pk 0.2 90 50 1-2 days
$212.16
1370006 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 90mm, 50/Pk 0.45 90 50 1-2 days
$212.16
1370019 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 300 x 3000mm, 1/Pk 0.45 300x3000 1 1-2 days
$804.31
1370018 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 293mm, 25/Pk 0.45 293 25 1-2 days
$655.50
1370017 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 142mm, 50/Pk 0.45 142 50 1-2 days
$448.38
1370016 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 47mm, 100/Pk 0.45 47 100 1-2 days
$181.43
1370015 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 25mm, 100/Pk 0.45 25 100 1-2 days
$138.67
1370014 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.45 Micron, 13mm, 100/Pk 0.45 13 100 1-2 days
$114.41
1370013 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 300 x 3000mm, 1/Pk 0.2 300x3000 1 1-2 days
$804.31
1370012 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 293mm, 25/Pk 0.2 293 25 1-2 days
$655.50
1370011 Polyvinylidene Flouride (PVDF) Membrane Filters, 0.2 Micron, 142mm, 50/Pk 0.2 142 50 1-2 days
$448.38
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PVDF Membrane Filter Applications

  • Aqueous and compatible organic HPLC samples and mobile phases
  • Filtration/sterilization of proteinaceous aqueous solutions (culture media or cell  lysate)
  • Filtration of aqueous solutions (strong acids, bases, and oxidizers) and compatible solvents (alcohols, aromatic hydryocarbons)

PVDF Membrane Filter Specifications

General

USP Class VI Testing Passed
BSA Protein Binding ~4 μg/cm2
Sterilization Gamma Irradiation, EtO, Autoclave
Max. Operating Temp 85 °C (185 °F)

Performance by Pore Size

  Thickness (µm) Water Flow Rate¹ Bubble Point (psi)
0.22 µm 125 7 56
0.45 µm 115 29 25
1. Measured with water in mL/min/cm2 at 13.5 psi

Documentation

Frequently Asked Questions


The pores of microporous membrane filters act as small capillaries.  When hydrophilic membranes come into contact with water, capillary action associated with surface tension forces causes the water to spontaneously enter and fill the pores.  In this manner, the membranes are easily wetted and allow the bulk flow of water through the pores.  Once wetted, hydrophilic membranes will not allow the bulk flow of air or other gasses, unless they are applied at pressures greater than the membrane’s bubble point.

Hydrophilic membrane filters are typically used with water and aqueous solutions.  They can also be used with compatible non-aqueous fluids.  Hydrophilic membrane filters are typically not used for air, gas or vent filtration since the filters would block flow if inadvertently wetted, by condensation for example.

When hydrophobic membranes come into contact with water, surface tension forces act to repel the water from the pores.  Water will not enter the pores and the membranes will act as a barrier to water flow, unless the water is applied at pressures greater than the membrane’s water entry pressure.  Low surface tension fluids, such as alcohols, can spontaneously enter and fill the pores of hydrophobic membranes.  Once all the air in the pores is displaced, there are no longer any surface tension forces and water can easily enter the pores, displace the low surface tension fluid, and pass through the membrane.  The membrane will then allow bulk flow of water for as long as the pore remain water filled.  If the membrane is allowed to dry (i.e. air enters the pores), then it must be pre-wet with a low surface tension fluid again prior to use with water.

Hydrophobic membrane filters are typically used with compatible non-aqueous fluids.  They are also commonly used as air, gas, or vent filters.  Hydrophobic membrane filters are sometimes used with water or aqueous solutions; and, in these applications, they must first be prewet with a low surface tension, water miscible fluid prior to use.

Q. What is the difference between nominal and absolute pore size ratings?

Nominal pore size ratings are provided as a general indication of filter retention.  It is understood that some quantity of particles greater than, and equal to, the nominal pore size ratings will pass through the filters into the filtrate.  Some manufactures may associate nominal pore size ratings with percentage filtration efficiencies. Nominal pore size ratings vary from manufacturer to manufacturer and, consequently, are not necessarily equivalent. Filters from different manufactures with similar nominal pore size ratings may not actually exhibit similar retention characteristics.

Absolute pore size ratings are typically based on retention studies performed using challenge suspensions of standard microorganism cultures or particles of known size. Absolute pore size ratings represent the size of the smallest microorganisms or particles completely retained during these studies. Absolute pore size ratings are almost always correlated to bubble point specifications that are used for quality control during membrane manufacturing. For the most part, absolute pore size ratings, especially those based on microbial retention, are comparable from manufacturer to manufacturer. There is more uncertainty for absolute pore size ratings based on particle retention studies, especially for pore size ratings <0.2µm, since there are no standard methods for these studies.

Regardless of pore size ratings, it is important to understand that application conditions do influence particle retention. Even filters with absolute pore size ratings can be operated in conditions that will allow unexpectedly sized particles to pass.


The pore size refers to the diameter of the individual pores in a membrane filter.   Pore size is typically specified in micrometers (µm).   Most membranes and filter media actually contain a distribution of pore sizes.  Nominal pore size ratings typically refer to the predominant pore size of a filtration media; pores larger and smaller than the nominal rating may be present.  Absolute pore size ratings typically refer to the largest pore size of a membrane and it is expected that all pores will be equal to or smaller than the absolute rating.

For the polycarbonate track-etch (PCTE) and polyester track-etch (PETE) membrane filters, porosity is the percent of the total surface area occupied by the pores; it typically ranges from <1% to 16%.  For the other membrane filters, porosity is the percent of the total volume occupied by the pores; it typically ranges from 40 to 80%.

You can find the Sterlitech compatibility guide.  It is important to realize that application conditions, such as operating temperature, affect compatibility.  Please contact us at [email protected] if you need assistance.

The bubble point is the minimum amount of pressure required to push air bubbles through the largest pore of a wet membrane.  The bubble point is inversely proportional to the pore diameter, as the pore diameter decreases the bubble point increases and vice versa.

Retention efficiency of membrane filters can be directly measured by challenging the filters with suspensions of standard microorganism cultures or particles of known size.  Unfortunately, such efficiency testing is necessarily destructive.  However, since retention characteristics are dependent on pore size, it is possible to correlate destructive challenge testing results to non-destructive membrane bubble point tests.  In this manner, the relationship between membrane pore size and membrane bubble point is empirically determined.  Typically, a minimum bubble point can be determined and specified for a particular pore size rating.  The bubble point specification is then used for quality control during membrane manufacture.  The bubble point can also be used by the consumer as a nondestructive test to verify membrane integrity before and/or after use.    

Depth filters are constructed with relatively thick filtration media and typically have nominal pore size ratings >1µm. Due to their large void volume, they capture significant amounts of particulate within their pore structure.
Membrane filters are typically composed of polymers that have been chemically processed, resulting in highly porous thin films with microscopic pore structures. Membrane filters typically have absolute pore size ratings <1µm, with some exceptions. Because of their very fine pore structure, membrane filters tend to trap the majority of particles on the surface. However, smaller particles with diameters near or below the pore size rating can be captured within the membrane or pass through the membrane.

membrane filters vs separator papers

To ensure ease of use, the membrane filters as stacked in their packaging are interleafed with layers of separator paper.  In most cases, the membrane filters will be white in color except for the track-etch membranes which are colorless and translucent.  In some special cases, the membranes will be dyed dark grey to black in appearance.  In all cases, the separator paper will be a different color than the membrane and is usually not white.  Please contact us at [email protected] if you need assistance.