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Frequently Asked Questions

Cellulose Acetate (CA) Membranes FAQ

<p><span data-sheets-value="{&quot;1&quot;:2,&quot;2&quot;:&quot;Yes, the cellulose acetate (CA) membranes contain a proprietary surfactant.\u00a0 The surfactant is used to ensure the CA membrane filters have good wettability.&quot;}" data-sheets-userformat="{&quot;2&quot;:9089,&quot;3&quot;:{&quot;1&quot;:0},&quot;10&quot;:0,&quot;11&quot;:4,&quot;12&quot;:0,&quot;16&quot;:8}">Yes, the cellulose acetate (CA) membranes contain a proprietary surfactant.&nbsp; The surfactant is used to ensure the CA membrane filters have good wettability.</span></p>
<p><span data-sheets-value="{&quot;1&quot;:2,&quot;2&quot;:&quot;Cellulose acetate (CA) membranes are made of entirely cellulose acetate polymer. \u00a0These membrane filters have the lowest protein binding of the filer types in question and typically have the greatest throughput when used with proteinaceous solutions.\u00a0 They are also used in applications where maximum protein recovery is critical.\u00a0The Sterlitech CA membranes have an integral nonwoven polyester support layer for improved strength and handling ease. \n\nIdeally, nitrocellulose (NC) membranes would be made entirely of nitrocellulose polymer. However, almost all commercially available filtration grade membrane filters labeled as \u201cnitrocellulose\u201d are actually composed of membranes made with a mixture of nitrocellulose and cellulose acetate polymers.\u00a0 \u00a0This is because pure nitrocellulose membrane filters are very difficult to manufacture with accpetable characteristics.\u00a0In an effort to provide\u00a0clarity to consumers, some manufactures refer to membranes containing a mixture of nitrocellulose and cellulose acetate polymers as mixed cellulose esters (MCE) membranes.\u00a0 In almost all applications, commercial nitrocellulose (NC) membrane filters and mixed cellulose esters (MCE) membrane filters are equivalent.\n\nMixed cellulose esters (MCE) membrane filters do not have a discrete support layer and are somewhat fragile and more difficult to handle\nwhen compared to CA membrane filters.\u00a0 MCE membranes exhibit greater protein binding than CA membranes. \u00a0However, MCE membranes tend to have more uniform pore structure and intrinsically greater clean water flow.\u00a0 MCE membrane filters have very good biocompatibility and are commonly used for microbiological studies.\u00a0 MCE filters are also traditionally used for silt density index (SDI) measurments.\n\nRegenerated cellulose (RC) membranes are made by chemically treating nitrocellulose membranes so that the material reverts back to natural\ncellulose.\u00a0 RC filters have the very good solvent resistance of traditional filter papers but with the much finer retention characteristics of polymer membrane filters. \u00a0\u00a0Additionally, regenerated cellulose is inherently hydrophilic and RC filters do not contain the wetting agents required by MCE and CA membrane filters.\n\n&quot;}" data-sheets-userformat="{&quot;2&quot;:9089,&quot;3&quot;:{&quot;1&quot;:0},&quot;10&quot;:0,&quot;11&quot;:4,&quot;12&quot;:0,&quot;16&quot;:8}">Cellulose acetate (CA) membranes are made of entirely cellulose acetate polymer. &nbsp;These membrane filters have the lowest protein binding of the filer types in question and typically have the greatest throughput when used with proteinaceous solutions.&nbsp; They are also used in applications where maximum protein recovery is critical.&nbsp;The Sterlitech CA membranes have an integral nonwoven polyester support layer for improved strength and handling ease. <br /><br />Ideally, nitrocellulose (NC) membranes would be made entirely of nitrocellulose polymer. However, almost all commercially available filtration grade membrane filters labeled as &ldquo;nitrocellulose&rdquo; are actually composed of membranes made with a mixture of nitrocellulose and cellulose acetate polymers.&nbsp; &nbsp;This is because pure nitrocellulose membrane filters are very difficult to manufacture with accpetable characteristics.&nbsp;In an effort to provide&nbsp;clarity to consumers, some manufactures refer to membranes containing a mixture of nitrocellulose and cellulose acetate polymers as mixed cellulose esters (MCE) membranes.&nbsp; In almost all applications, commercial nitrocellulose (NC) membrane filters and mixed cellulose esters (MCE) membrane filters are equivalent.<br /><br />Mixed cellulose esters (MCE) membrane filters do not have a discrete support layer and are somewhat fragile and more difficult to handle<br />when compared to CA membrane filters.&nbsp; MCE membranes exhibit greater protein binding than CA membranes. &nbsp;However, MCE membranes tend to have more uniform pore structure and intrinsically greater clean water flow.&nbsp; MCE membrane filters have very good biocompatibility and are commonly used for microbiological studies.&nbsp; MCE filters are also traditionally used for silt density index (SDI) measurments.<br /><br />Regenerated cellulose (RC) membranes are made by chemically treating nitrocellulose membranes so that the material reverts back to natural<br />cellulose.&nbsp; RC filters have the very good solvent resistance of traditional filter papers but with the much finer retention characteristics of polymer membrane filters. &nbsp;&nbsp;Additionally, regenerated cellulose is inherently hydrophilic and RC filters do not contain the wetting agents required by MCE and CA membrane filters.<br /><br /></span></p>

Cellulose Acetate (CA) membrane filters are among the lowest protein-binding membranes available, making them ideal for applications requiring maximum protein recovery. They provide high throughput with protein-rich solutions and are commonly used for filtering proteins, enzymes, tissue culture media, serums, and biological fluids.

CA membranes feature an integral nonwoven polyester (PET) support layer, offering strength, dimensional stability, and easy handling, with resistance to tearing and curling. They are naturally hydrophilic, wet easily with aqueous solutions, offer good chemical resistance, are compatible with low molecular weight alcohols, and can withstand steam sterilization up to 135 °C.

Excluding the polyester support layer, Cellulose Acetate (CA) membrane filters are composed entirely of cellulose acetate polymer. There may be a very small amount of residual lignin present as a trace component. This minimal lignin content does not affect the performance, purity, or reliability of CA membrane filters in typical laboratory, analytical, and sample filtration applications.

Sterlitech Cellulose Acetate (CA) membranes are made from cellulose diacetate. These membrane filters also have an integral nonwoven polyester (polyethylene terephthalate) support layer. When evaluating application compatibility, both materials should be considered.