Frequently Asked Questions

We have a Chemical Compatibility Chart that you can use for reference. 

Yes, there is. You can view the bibliography for cellQART® here.

Almost all cells do best in a tight range of incubation parameters (temperature, CO2 concentration, media condition, humidity, amount of light, etc.). Keep deviation to a minimum.

Many in the field put a Matrigel or other firm coating on the membrane to better observe cell invasion to the other (bottom) side.

The higher porosity of translucent membranes allows for easier cell migration and, consequently, more observations.

Yes, it seems that 3 µm – 8 µm pore sizes are best suited for these studies; but it is critical to check cell size first.

Experiment with two (2) or more densities lower and higher than the current value. Proceed in the direction of improved results.

Yes! When mounting on both sides of the inserts, first mount cells on the bottom side.

While our membranes are tissue-culture treated to stimulate adherence, some cell types benefit from additional coatings that facilitate growth.

Prior to seeding, incubate the inserts with the intended media for at least 1 hour at the desired temperature

Transparency increases as porosity decreases, so porosity requirements will impact transparency.

Either of our transparent or translucent membranes can be used for fluorescent analysis.

Our transparent membranes are better suited for phase contrast and live monitoring.

cellQART^® inserts are polyester (polyethylene terephthalate) track-etch membrane. This difference will likely influence how they grow. We recommend testing different seeding densities that are higher and lower than your current method and proceed in the direction of improved results.