Page 14 - Filtration Resources

When counting bacteria as part of epifluorescent microscopy we generally recommend using the black polycarbonate membranes instead of cellulose membranes. This is because the black polycarbonate materials have a uniform pore size and flat surface that will retain all of the bacteria without trapping any inside of the filter. Though cellulose membranes will retain bacteria, it often will become trapped inside of the filter, where it cannot be counted.

Recently one of our customers was interested in testing Legionella bacteria and asked us how our polycarbonate membranes fit into the process mentioned on our website. If you are unfamiliar with Legionella, it is a waterborne pathogen commonly found in aerosolized waters such as cooling towers, showers, and humidifiers, and it is best known as the cause of Legionnaire’s Disease as well as Pontiac Fever. Its name originated from an outbreak that occurred at the 1976 convention of the American Legion in Philadelphia.

There are actually two areas in which membranes are used in regards to Legionella: Sample preparation and point-of-use filtration. For sample preparation the CDC (Centers for Disease Control) recommends using a 0.2 micron, 47mm polycarbonate filter to extract Legionella bacterium from potable water. Non potable water utilizes a direct plating procedure.

Point of use filtration frequently involves a device that attaches to a faucet or showerhead to eliminate Legionella. Such devices

Here is an article that does a great job of explaining what efficiency ratings mean on a filter and how they are calculated, courtesy of the American Filtration & Separation Society.  This is very useful information for filter users and purchasing agents on the practical effects the filter efficiency will have in a real world setting. You can read the whole thing here.

Over the years we have seen an increased use of filtration equipment in juice processing, particularly regarding ultrafiltration (UF) or microfiltration (MF)for the clarification of apple juice.  Since it has been demonstrated that membrane filtration can produce yields of 95%-99% - compared to only 80-94% through conventional processes – it is no wonder that filtration methods are growing in prevalence.  The greater yield combined with the reduced time and labor costs have translated to hundreds of thousands of dollars saved for juice processing plants! If you are considering juice filtration, here a couple of tips to keep in mind:

• The juice must be clear.  Of the four common types of apple juice produced – natural, crushed, clarified, and clear – only clear juice is suitable for membrane processing.
• Consider ceramic membranes.  More and more fruit juice installations are installing ceramic membranes.  While these do have  a higher cost than other materials, they do offer

One of the important characteristics in membrane selection is whether you want a membrane that is Hydrophobic or Hydrophilic. Here we'll define these terms, as well as provide some examples of membrane materials and applications for both types. Hydrophilic literally means “water loving.” Hydrophilic membranes will attract water, and in the process push away other molecules in order to allow water access to the membrane. This keeps contaminants away from the membrane allowing it stay clean and functioning for a longer period of time. Because of this trait hydrophilic membranes are especially well suited for medical applications and biological assays. Hydrophobic on the other hand, literally means “afraid of water.” These membranes will block the passage of water and are commonly used for applications involving separation of water from other materials, such as venting gases.

From time to time we hear from customers that they have "scratched" their silver membranes.  This occasionally leads them to ask: Is there some sort of coating on the membrane that is being rubbed off?

Answer: Our silver membranes are made of 99.97% pure silver and do not have any coating on them.  What may appear to be scratching is actually a polishing of the silver surface, which makes the silver reflective.  This can be done when the membrane is rubbed with a metal object, such as tweezers.  We also posted a brief video demonstrating the effect.  You can watch it here.