Cell Migration Studies Using cellQART® Cell Culture Inserts
Cell Migration Studies Using cellQART® Cell Culture Inserts
Cell migration plays a critical role in various biological processes, including tissue homeostasis, cancer metastasis, and immune responses. Researchers studying these processes require reliable tools to observe and quantify cell movement under controlled conditions. In the cell migration assay, cells migrate through the semipermeable membrane of a cellQART® Cell Culture Insert in response to specific stimuli.
How cellQART® Cell Culture Inserts Support Migration Assays
cellQART® Cell Culture Inserts are designed with a microporous membrane that allows for the separation of two compartments, enabling researchers to study directional cell movement. The membrane pore size and material can be selected based on the cell type and experimental needs, ensuring optimal assay performance.
Pore Size Recommendations for Migration Assays:
| Cell Type | Pore Size Recommendation (µm) |
| Endothelial, HUVEC, HMVEC | 3.0 |
| Neutrophils, PMNs | 3.0 |
| Lymphocytes, macrophages, monocytes | 3.0, 5.0 |
| Neuronal cells | 3.0 |
| Dendritic cells | 3.0, 5.0, 8.0 |
| Neurite outgrowth | 1.0, 3.0 |
| Epithelial fibroblasts | 8.0 |
| Leukocytes | 3.0, 5.0 |
| Smooth muscle | 8.0 |
Key Advantages of Using cellQART® for Migration Studies
- Versatility: Compatible with various cell types, including epithelial, endothelial, neutrophils, and leukocytes
- High-Quality Membranes: Available in different pore sizes (1.0 µm, 3.0 µm, 5.0 µm, 8.0 µm) to accommodate different cell migration studies.
- Transparent Options: Facilitates real-time observation of cell movement.
- Ease of Use: Simple setup and compatibility with standard multi-well plates.
cellQART® cell culture inserts provide a reliable, flexible, and efficient platform for cell migration studies. By offering customizable options and consistent performance, they empower researchers to gain deeper insights into cellular behavior in various biological processes. Whether for basic research or drug development, cellQART® inserts contribute to advancing scientific discoveries in cell migration.
Scientific Papers Citing cellQART® for Migration Assays:
​Xu Y, Cohen E, Johnson CN, Parent CA, Coulombe PA. Repeated stress to the skin amplifies neutrophil infiltration in a keratin 17- and PKCα-dependent manner. PLoS Biol. 2024 Aug;22(8):e3002779. doi: 10.1371/journal.pbio.3002779. PMID: 39159283. Available from: https://www.lifescience.net/publications/976900/repeated-stress-to-the-skin-amplifies-neutrophil-i/
Dacheux, M. A. et al. Prometastatic Effect of ATX Derived from Alveolar Type II Pneumocytes and B16-F10 Melanoma Cells. Cancers 14, 1586 (2022). Available from: https://www.mdpi.com/2072-6694/14/6/1586
