Aluminum Oxide Membrane Filters, 0.2 Micron, 25mm, 50/pk
Alumina Oxide Membrane Filter Specifications
Applications:
- HPLC sample and mobile phase filtration
- Filtration of aggressive organic solvents
- Virus concentration and analysis
- Analysis of micro plastic in water
- Epifluorescence microscopy
- Synthesis of nanostructures (e.g. nanotubes)
Features:
- Fast filtration, efficient separation and high purity
- High number of tightly controlled pores in honeycomb shape
- Free of organic extractables and leachables
- Minimum adsorption
- Transparent when wet
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Frequently Asked Questions
Q: What is the difference between hydrophilic and hydrophobic membrane filters?
The pores of microporous membrane filters act as small capillaries. When hydrophilic membranes come into contact with water, capillary action associated with surface tension forces causes the water to spontaneously enter and fill the pores. In this manner, the membranes are easily wetted and allow the bulk flow of water through the pores. Once wetted, hydrophilic membranes will not allow the bulk flow of air or other gasses, unless they are applied at pressures greater than the membrane’s bubble point.
Hydrophilic membrane filters are typically used with water and aqueous solutions. They can also be used with compatible non-aqueous fluids. Hydrophilic membrane filters are typically not used for air, gas or vent filtration since the filters would block flow if inadvertently wetted, by condensation for example.
When hydrophobic membranes come into contact with water, surface tension forces act to repel the water from the pores. Water will not enter the pores and the membranes will act as a barrier to water flow, unless the water is applied at pressures greater than the membrane’s water entry pressure. Low surface tension fluids, such as alcohols, can spontaneously enter and fill the pores of hydrophobic membranes. Once all the air in the pores is displaced, there are no longer any surface tension forces and water can easily enter the pores, displace the low surface tension fluid, and pass through the membrane. The membrane will then allow bulk flow of water for as long as the pore remain water filled. If the membrane is allowed to dry (i.e. air enters the pores), then it must be pre-wet with a low surface tension fluid again prior to use with water.
Hydrophobic membrane filters are typically used with compatible non-aqueous fluids. They are also commonly used as air, gas, or vent filters. Hydrophobic membrane filters are sometimes used with water or aqueous solutions; and, in these applications, they must first be prewet with a low surface tension, water miscible fluid prior to use.
Q: Can I use the aluminum oxide (AO) membrane disk filters in the stirred cells?
Yes, you can use the AO membrane disc filters in the stirred cells. These disk filters are very brittle and must be handled with an abundance of care to avoid breakage. Use membrane tweezers to carefully place the disk filter on the surface of the support disk and then install the support disk in the stirred cell body.
Q: Aluminum Oxide (AO) Handling Tips
AO Membranes are extremly brittle. Click here to see our blog for handling the membranes
Q: What are the advantages of Aluminum Oxide membrane filters?
Aluminum oxide (anodic aluminum oxide, AAO) membrane filters are free of organic extractables and leachables, making them ideal for high-purity filtration. They exhibit very low nonspecific adsorption, helping preserve sample integrity and maximize analyte recovery. Their inorganic structure provides excellent chemical and thermal stability, along with uniform pore size and high porosity, supporting reliable, efficient filtration in analytical and research applications.
Q: Are the aluminum oxide membrane disk filters available in diameters not listed in the ordering information?
Unfortunately, we are unable to supply the aluminum oxide membrane filters with custom diameters. Please contact us at [email protected] to inquire about alternatives.
Q: Are the aluminum oxide membrane filters asymmetric and, if so, which surface has the smallest pores?
Yes, the aluminum oxide membrane filters are somewhat asymmetric. The shiny side of the filter is the surface with the smallest pores. The shiny side faces up in the packaging.
Q: What is the difference between nominal and absolute pore size ratings?
Nominal pore size ratings provide a general indication of filter retention efficiency, meaning some particles equal to or larger than the stated pore size may pass through the filter. Nominal ratings can vary by manufacturer, so filters with the same nominal pore size may not offer equivalent filtration performance.
Absolute pore size ratings are determined through controlled particle or microbial retention testing and represent the smallest particles that are consistently retained by the membrane. These ratings are often correlated with bubble point specifications and are generally more comparable across manufacturers.
Important: Actual filtration performance depends on application conditions, even when using filters with absolute pore size ratings.
Q: Are the aluminum oxide membrane filters available with a perimeter support ring?
The Sterlitech aluminum oxide membrane filters are not available with perimeter support rings. However, when handled with care, they are suitable alternatives to competitive aluminum oxide membrane filters with ring supports.
Q: What is the difference between pore size and porosity?
The pore size refers to the diameter of the individual pores in a membrane filter. Pore size is typically specified in micrometers (µm). Most membranes and filter media actually contain a distribution of pore sizes. Nominal pore size ratings typically refer to the predominant pore size of a filtration media; pores larger and smaller than the nominal rating may be present. Absolute pore size ratings typically refer to the largest pore size of a membrane and it is expected that all pores will be equal to or smaller than the absolute rating.
For the polycarbonate track-etch (PCTE) and polyester track-etch (PETE) membrane filters, porosity is the percent of the total surface area occupied by the pores; it typically ranges from <1% to 16%. For the other membrane filters, porosity is the percent of the total volume occupied by the pores; it typically ranges from 40 to 80%.
Q: What can be used to dissolve the aluminum oxide membrane filters?
The aluminum oxide membrane filters can be readily dissolved with sodium hydroxide solutions at 1M concentration or higher. The time required to dissolve the membrane can be reduced by elevating the temperature and/or increasing the concentration.
Q: How do I determine if my filter is compatible with my application?
You can find the Sterlitech compatibility guide. It is important to realize that application conditions, such as operating temperature, affect compatibility. Please contact us at [email protected] if you need assistance.
Q: Can the aluminum oxide membrane filters be used as alternatives for the Whatman Anodisc filters?
Yes. Sterlitech aluminum oxide (AAO) membrane filters can be used as alternatives to Whatman Anodisc filters. Please note that Sterlitech alumina membrane filters do not have perimeter support rings, so additional care is recommended during handling to prevent damage.
Q: What is a bubble point and how is it determined?
The bubble point is the minimum amount of pressure required to push air bubbles through the largest pore of a wet membrane. The bubble point is inversely proportional to the pore diameter, as the pore diameter decreases the bubble point increases and vice versa.
Retention efficiency of membrane filters can be directly measured by challenging the filters with suspensions of standard microorganism cultures or particles of known size. Unfortunately, such efficiency testing is necessarily destructive. However, since retention characteristics are dependent on pore size, it is possible to correlate destructive challenge testing results to non-destructive membrane bubble point tests. In this manner, the relationship between membrane pore size and membrane bubble point is empirically determined. Typically, a minimum bubble point can be determined and specified for a particular pore size rating. The bubble point specification is then used for quality control during membrane manufacture. The bubble point can also be used by the consumer as a nondestructive test to verify membrane integrity before and/or after use.
Q: What is the difference between a depth filter and membrane filter?
Depth filters are constructed with relatively thick filtration media and typically have nominal pore size ratings >1µm. Due to their large void volume, they capture significant amounts of particulate within their pore structure.
Membrane filters are typically composed of polymers that have been chemically processed, resulting in highly porous thin films with microscopic pore structures. Membrane filters typically have absolute pore size ratings <1µm, with some exceptions. Because of their very fine pore structure, membrane filters tend to trap the majority of particles on the surface. However, smaller particles with diameters near or below the pore size rating can be captured within the membrane or pass through the membrane.
Q: What's the benefit of purchasing sample packs?
Sample packs allow the customer to purchase small quantities of membrane filters at nominal cost, with various diameters and pore sizes as selected. This allows the customer to preform trials as needed to determine the optimal filter for their application before committing to purchasing standard pack quantities.
Q: Are membrane filter samples available in diameters others than 13, 25, or 47mm?
In most cases, membrane filter samples can be purchased in sizes that are not listed in the standard sample packs. Please contact us at [email protected] to inquire about availability and pricing.
Q: How can I tell the difference between the separator papers and the membrane filters?
To ensure ease of use, the membrane filters as stacked in their packaging are interleafed with layers of separator paper. In most cases, the membrane filters will be white in color except for the track-etch membranes which are colorless and translucent. In some special cases, the membranes will be dyed dark grey to black in appearance. In all cases, the separator paper will be a different color than the membrane and is usually not white. Please contact us at [email protected] if you need assistance.
Q: What filters can be used for infrared spectroscopy applications where the retained particles are analyzed while resting on the filter surface?
- Silver Membrane Filters: These are the best choice for reflectance IR spectroscopy, but cannot be used for transmission IR spectroscopy.
- Aluminum Oxide Membrane Filters: Depending on the spectral range, these are a good choice for transmission IR spectroscopy, but they are a poor choice for reflectance IR spectroscopy. These filters are extremely brittle and will fracture if subjected to bending. They cannot be handled with fingers; membrane tweezers must be used.
- Glass Microfiber Filters: Depending on the spectral range, these filters can be used for both reflectance and transmission IR spectroscopy. The paper does not specify any particular pore sizes; however, most of our offerings should have good efficiency with ≥5-micron sized particles. For good light transmission, I would guess that a thin, binder- less filter media would be best, such as Grade C or GC-50. Gold-Coated Polyester Track-Etch Filters: Gold coated polyester track-etch filters have essentially the same spectral properties as gold-coated polycarbonate track-etch filters; but they are inherently hydrophilic and, consequently, much more convenient for use with water samples. These are a good choice for reflectance IR spectroscopy, but cannot be used for transmission IR spectroscopy.




