Polyethersulfone (PES) Membrane Filters, 0.1 Micron, 200x200mm, 5/Pk

Sterlitech PES (polyethersulfone) membrane filters, 0.1 micron pore size, 200 x 200 mm sheets, pack of 5. Hydrophilic, low protein/drug binding, minimal extractables, and broad chemical tolerance; ideal for pharmaceutical, medical, and life science applications.
  • SKU
    PES012005
  • Pore Size (µm)
    0.10µm
  • Diameter (mm)
    200 x 200
  • Pack Size
    5
$123.48
+ -

Polyethersulfone (PES) Membrane Filters

Ideal for tissue culture media sterilization, life science and microbiology fluid applications, clinical, and general filtration.


  • Hydrophilic water compatibility: Eliminates the analytical interference of wetting agents
  • Low extractables: Ensures test results will not be compromised
  • Low drug and protein binding: Maximizes recovery of critical drugs, proteins, and enzymes
  • Wide range of pore sizes: Pore sizes range from 0.03 to 5.0 μm, accommodating a multitude of users and applications
  • Superior burst strength: Protects the integrity of the membrane under high pressure
  • Lot-to-lot consistency: Quality checks, both down and across the membrane, ensure dependable results every time

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Or call us at 1-877-544-4420

PES Membrane Filter Applications

  • Blood Glucose Testing
  • Drug Filtration
  • I.V. Filters
  • Lateral Flow Assays
  • Particulate Removal
  • Serum Cholesterol Testing

 

PES Membrane Filter Specifications

General

 Sterilization   Gamma Irradiation, EtO, Autoclave, Live Steam
 USP Class VI Testing   Passed
 BSA Protein Binding   ~20 μg/cm2 
 Extractables   <2%
 Max. Operating Temp   130 °C (266 °F)
 Sealing Compatibility   Ultrasonic, Heat, Radio Frequency, and Insert Molding
 Nominal Thickness   110-150 µm

 

Performance by Pore Size

  Flow Rate1 Bubble Point (psi)
 0.03 µm 5.5 90.0
 0.10 µm 11.7 70.0
 0.20 µm 33.2 50.0
 0.45 µm 58.2 35.0
 0.65 µm 95.5 21.3
 0.80 µm 117.0 13.0
 1.20 µm 143.0 11.0
 5.00 µm 186.0 6.0
*Notes:
1.
Flow rate measured with water as mL/min/cm2 at 10 psi (0.7 kg/cm2)

The pores of microporous membrane filters act as small capillaries.  When hydrophilic membranes come into contact with water, capillary action associated with surface tension forces causes the water to spontaneously enter and fill the pores.  In this manner, the membranes are easily wetted and allow the bulk flow of water through the pores.  Once wetted, hydrophilic membranes will not allow the bulk flow of air or other gasses, unless they are applied at pressures greater than the membrane’s bubble point.

Hydrophilic membrane filters are typically used with water and aqueous solutions.  They can also be used with compatible non-aqueous fluids.  Hydrophilic membrane filters are typically not used for air, gas or vent filtration since the filters would block flow if inadvertently wetted, by condensation for example.

When hydrophobic membranes come into contact with water, surface tension forces act to repel the water from the pores.  Water will not enter the pores and the membranes will act as a barrier to water flow, unless the water is applied at pressures greater than the membrane’s water entry pressure.  Low surface tension fluids, such as alcohols, can spontaneously enter and fill the pores of hydrophobic membranes.  Once all the air in the pores is displaced, there are no longer any surface tension forces and water can easily enter the pores, displace the low surface tension fluid, and pass through the membrane.  The membrane will then allow bulk flow of water for as long as the pore remain water filled.  If the membrane is allowed to dry (i.e. air enters the pores), then it must be pre-wet with a low surface tension fluid again prior to use with water.

Hydrophobic membrane filters are typically used with compatible non-aqueous fluids.  They are also commonly used as air, gas, or vent filters.  Hydrophobic membrane filters are sometimes used with water or aqueous solutions; and, in these applications, they must first be prewet with a low surface tension, water miscible fluid prior to use.

Q. What is the difference between nominal and absolute pore size ratings?

Nominal pore size ratings are provided as a general indication of filter retention.  It is understood that some quantity of particles greater than, and equal to, the nominal pore size ratings will pass through the filters into the filtrate.  Some manufactures may associate nominal pore size ratings with percentage filtration efficiencies. Nominal pore size ratings vary from manufacturer to manufacturer and, consequently, are not necessarily equivalent. Filters from different manufactures with similar nominal pore size ratings may not actually exhibit similar retention characteristics.

Absolute pore size ratings are typically based on retention studies performed using challenge suspensions of standard microorganism cultures or particles of known size. Absolute pore size ratings represent the size of the smallest microorganisms or particles completely retained during these studies. Absolute pore size ratings are almost always correlated to bubble point specifications that are used for quality control during membrane manufacturing. For the most part, absolute pore size ratings, especially those based on microbial retention, are comparable from manufacturer to manufacturer. There is more uncertainty for absolute pore size ratings based on particle retention studies, especially for pore size ratings <0.2µm, since there are no standard methods for these studies.

Regardless of pore size ratings, it is important to understand that application conditions do influence particle retention. Even filters with absolute pore size ratings can be operated in conditions that will allow unexpectedly sized particles to pass.


Depth filters are constructed with relatively thick filtration media and typically have nominal pore size ratings >1µm. Due to their large void volume, they capture significant amounts of particulate within their pore structure.
Membrane filters are typically composed of polymers that have been chemically processed, resulting in highly porous thin films with microscopic pore structures. Membrane filters typically have absolute pore size ratings <1µm, with some exceptions. Because of their very fine pore structure, membrane filters tend to trap the majority of particles on the surface. However, smaller particles with diameters near or below the pore size rating can be captured within the membrane or pass through the membrane.

The pore size refers to the diameter of the individual pores in a membrane filter.   Pore size is typically specified in micrometers (µm).   Most membranes and filter media actually contain a distribution of pore sizes.  Nominal pore size ratings typically refer to the predominant pore size of a filtration media; pores larger and smaller than the nominal rating may be present.  Absolute pore size ratings typically refer to the largest pore size of a membrane and it is expected that all pores will be equal to or smaller than the absolute rating.

For the polycarbonate track-etch (PCTE) and polyester track-etch (PETE) membrane filters, porosity is the percent of the total surface area occupied by the pores; it typically ranges from <1% to 16%.  For the other membrane filters, porosity is the percent of the total volume occupied by the pores; it typically ranges from 40 to 80%.

You can find the Sterlitech compatibility guide posted at https://www.sterlitech.com/pub/media/pdf_resources/Chemical_Compatibility.pdf.  It is important to realize that application conditions, such
as operating temperature, affect compatibility.  Please contact us at sales@sterlitech.com if you need assistance.

To ensure ease of use, the membrane filters as stacked in their packaging are interleafed with layers of separator paper.  In most cases, the membrane filters will be white in color except for the track-etch membranes which are colorless and translucent.  In some special cases, the membranes will be dyed dark grey to black in appearance.  In all cases, the separator paper will be a different color than the membrane and is usually not white.  Please contact us at sales@sterlitech.com if you need assistance.

The bubble point is the minimum amount of pressure required to push air bubbles through the largest pore of a wet membrane.  The bubble point is inversely proportional to the pore diameter, as the pore diameter decreases the bubble point increases and vice versa.

Retention efficiency of membrane filters can be directly measured by challenging the filters with suspensions of standard microorganism cultures or particles of known size.  Unfortunately, such efficiency testing is necessarily destructive.  However, since retention characteristics are dependent on pore size, it is possible to correlate destructive challenge testing results to non-destructive membrane bubble point tests.  In this manner, the relationship between membrane pore size and membrane bubble point is empirically determined.  Typically, a minimum bubble point can be determined and specified for a particular pore size rating.  The bubble point specification is then used for quality control during membrane manufacture.  The bubble point can also be used by the consumer as a nondestructive test to verify membrane integrity before and/or after use.    

PES Membranes are low protein binding, PES membrane filters are ideal for tissue culture media sterilization, life science and microbiology fluid applications. 

The polyethersulfone (PES) membrane filters have asymmetrical pore structure. The pore structure varies within the thickness of the membrane such that the largest openings occur on one side and the smallest openings occur on the opposite side. When viewing the membrane with reflected light at low incidence angles, each side has a somewhat different visual appearance. The side with the largest pores will appear more dull or matte than the side with the smallest pores. With a little bit of experience, most users can easily identify the sides. For optimal throughput, the PES membrane filters should be oriented so that side with the largest pores (the duller side) is facing upstream. For applications involving microscopic analyses of captured particles or microbes, the user may choose to orient the filter so that the side with the smallest pores (the shinier side) is facing upstream. This orientation may reduce throughput but it improves the likelihood of capturing particles of interest on the surface of the membrane instead of within the pore structure.

The polyethersulfone (PES) membranes used in the Sterlitech membrane filters have asymmetric pore structure.  The pore structure varies within the thickness of the membrane such that the largest openings occur on one side and the smallest openings occur on the opposite side.  When viewing the membrane with reflected light at low incidence angles, each side has a somewhat different visual appearance.  The side with the largest pores will appear more dull (or matte) than the side with the smallest pores
(which will appear shinier).  With a little bit of experience, most users can easily identify the sides.  The membranes can be used with either surface oriented upstream without affecting retention.  However, orienting the dull side upstream increases total throughput
while orienting the shiny side upstream allows for better analyses of the retained particles.

For lateral flow assays, membranes with high protein binding capacities are preferred.  Consequently, with their inherently low protein binding capacities, PES membrane filters are typically not used in these applications.