Peristaltic Pump Head, Sartorius VivaFlow, 1.6mm WT
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Recommended for processing samples with Vivaflow® tangential flow filtration (TFF) cassettes, these Sartorius pumps are ideal for ultrafiltration and diafiltration applications. In addition, they offer the flexibility, performance and control needed for other filtration and fluid transfer applications.
- Simple and Intuitive Set Up
Tool-free mounting of the pump head to the pump drive makes assembly a breeze, while tube loading is quick and easy with an intuitive and reliable retention system.
- Precise Flow Control
Digital control with optional remote input delivers accurate and consistent flow rates, and the instantly reversible motor allows for quick changes to the flow direction.
- Avoid Contamination
Only the tubing is in contact with the process fluid, eliminating the risk of cross-contamination between the pump and the sample, ensuring that both remain clean.
- Low Maintenance
Simply wipe down the pumps as part of your normal laboratory cleaning routine. Meanwhile, the washable pump heads only require occasional lubrication.
Compliance Information
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Certification
- ISO 9001
- ISO 14001
- ETL Listed
Dimensions
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Dimensions: 85 x 60 x 82 mm
General Specifications
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Flow Rate: 3-400 mL/min @ 3-400 rpm with 3.2 mm ID
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Tubing: 1.6 mm W.T.
Materials of Construction
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Materials of Construction
- Glass-filled polypropylene
- Polyarylamide
- Stainless steel
Applications
- Crossflow ultrafiltration and diafiltration
- Ion exchange and affinity chromatography
- In-line depth filtration and microfiltration
- Dispensing and filling
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Frequently Asked Questions
Q: What’s the best way to concentrate large sample volumes in one go?
Vivaflow® crossflow devices offer a fast, cost-effective way to concentrate 0.1–5 L samples without large-scale systems. With PES or Hydrosart® membranes and single-use or reusable options, they can achieve up to 50× concentration—1 L in ~30 min or 5 L in <75 min—with near-total recovery after a single buffer rinse.
Q: How can I reduce protein degradation during concentration?
To reduce protein degradation, optimize buffer composition and minimize shear stress—e.g., use lower centrifuge speeds for linear proteins and devices with membrane areas suited to your starting volume. Vivaflow® crossflow devices minimize shear compared to centrifugal or stirred cell systems, and diafiltration tools like the Vivaflow® reservoir help maintain optimal buffer conditions during concentration.
Q: Which ultrafiltration method is best suited for virus concentration?
For virus concentration, use a MWCO ~⅓ of the target’s molecular weight (or match pore size to diameter). For Lentivirus (~90 nm), a 300 kDa Hydrosart® membrane offers the best balance of speed and recovery, with 100 kDa PES as a slower alternative. Always validate device, membrane, and MWCO for your specific virus.
Q: How can I maximize my protein concentration and protein recovery?
To maximize protein concentration and recovery, match your membrane, MWCO, and device to your protein type. Consider sample properties, membrane material (PES, RC, CTA, HY), and choose a MWCO ~⅓ of target size. Optimize device design, pre-treat to reduce non-specific binding, and use dead-stop pockets or pre-filled filtrate tubes for maximum sample retrieval.
