HansaBioMed Hollow Fiber Module, Polysulfone, TFF-Easy
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TFF-Easy is a filter cartridge in hollow fibers made of polysulfone, which allows the concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification.
The small dimensions of the device allow to concentrate samples from 5 ml up to liters, surmounting the limit of the TFF technique which is usable for processing big volumes of fluids. The filter is additionally suitable for dialyzing EV preparations.
TFF-Easy can be easily washed and it is reusable multiple times.
Advantages: Washable, reusable multiple times, easy to use, fast concentration of EV containing matrices. EV dialysis.
| Sample type | Diluted EV containing matrices (i.e. urine, conditioned cell media) |
| Quantity | 1 concentrator |
| Filter cartridge | Polysulfone hollow fibres |
| Sample volume per reaction | Recommended sample volume 5-15 ml |
| EV recovery | 83% |
| Concentration time | 5 minutes |
| Reusable | Up to 20 times |
| Application |
Concentration of diluted matrices such as cell media or urine prior to EV isolation. Easy removal of small molecules and ions from the EV preparation. EV dialysis for changing buffer conditions. High efficiency of EV isolation if coupled with SEC columns. |
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Frequently Asked Questions
Q: What’s the best way to concentrate large sample volumes in one go?
Vivaflow® crossflow devices offer a fast, cost-effective way to concentrate 0.1–5 L samples without large-scale systems. With PES or Hydrosart® membranes and single-use or reusable options, they can achieve up to 50× concentration—1 L in ~30 min or 5 L in <75 min—with near-total recovery after a single buffer rinse.
Q: How can I reduce protein degradation during concentration?
To reduce protein degradation, optimize buffer composition and minimize shear stress—e.g., use lower centrifuge speeds for linear proteins and devices with membrane areas suited to your starting volume. Vivaflow® crossflow devices minimize shear compared to centrifugal or stirred cell systems, and diafiltration tools like the Vivaflow® reservoir help maintain optimal buffer conditions during concentration.
Q: Which ultrafiltration method is best suited for virus concentration?
For virus concentration, use a MWCO ~⅓ of the target’s molecular weight (or match pore size to diameter). For Lentivirus (~90 nm), a 300 kDa Hydrosart® membrane offers the best balance of speed and recovery, with 100 kDa PES as a slower alternative. Always validate device, membrane, and MWCO for your specific virus.
Q: How can I maximize my protein concentration and protein recovery?
To maximize protein concentration and recovery, match your membrane, MWCO, and device to your protein type. Consider sample properties, membrane material (PES, RC, CTA, HY), and choose a MWCO ~⅓ of target size. Optimize device design, pre-treat to reduce non-specific binding, and use dead-stop pockets or pre-filled filtrate tubes for maximum sample retrieval.
