Expired Sterile White MCE Membrane Filter, 0.45 micron, 47 mm Diameter

Our sterile, white Mixed Cellulose Ester (MCE) membranes--a versatile mixture of cellulose nitrate and cellulose acetate fibers--are general purpose filters with high recovery rates, high porosity, and superior flow rates. These pre-sterilized filters are suited for single use in many microbiological applications, including the examination of water, wastewater, pharmaceuticals, food, and beverages.
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Special Price$45.35was$90.70
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MCE Sterile Membrane Disc Filters

The filter of choice for water, wastewater, pharmaceutical, liquid food, and beverage single-use-applications.

  • Pre-Sterilized: For single-use applications
  • Hydrophilic water compatibility: For aqueous clarification and particulate capture
  • Consistent, high flow rates: Reduces process times with faster, more efficient filtration
  • Uniform pore structure and controlled thickness: Provides selectivity and consistency
  • Mixed, inert cellulose nitrate: For wide biological and chemical compatibility
  • Mixed cellulose acetate: Provides a smoother, more optically compatible surface than pure nitrocellulose
  • High protein binding capacity: Excellent retention of proteinaceous samples
  • High degree of internal surface area: Optimizes product adsorption


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MCE Sterile Membrane Filter Applications

Water and Wastewater Industry:
Capturing/culturing microorganisms using the MF Technique

  • Test Method 9222 B: Total Coliforms (0.45 µm)
  • Test Method 9222 D: Fecal Coliforms (0.45 µm)
  • Test Method 9230 C: Fecal Coliforms (0.45 µm)
  • Test Method 9215 D: Total Bacteria (0.20 µm/0.45 µm)
  • Test Method 9260 B: Salmonella (0.45 µm)
  • Test Method 9213: Pseudomonas sp (0.20 µm)
  • Test Method 9213 E: Psedomonas aeruginosa (0.80 µm)

MCE Membrane Filter Specifications


 USP Class VI Testing   Passed
 BSA Protein Binding   ~160 μg/cm2
 Extractables   <4%
 Max. Operating Temp   180 °C (356 °F)
 Sealing Compatibility   Ultrasonic, Heat, Radio Frequency, and Insert Molding
 Nominal Thickness   150 µm


Performance by Pore Size3

  Air Flow Rate1 Water Flow Rate2 Bubble Point (psi)
 0.10 µm 0.67 2.7 35.3
 0.20 µm 2.4 17.5 54.5
 0.45 µm 5.0 45.0 35.0
 0.65 µm 11.2 120.0 21.3
 0.80 µm 15.0 165.0 16.4
 1.00 µm 20.4 220.0 13.9
 3.00 µm 28.3 300.0 10.2
 5.00 µm 40.9 400.0 8.5
 8.00 µm 65.0 NA 4.0
Air flow rate measured as L/min/cm2
2. Water flow rate measured as mL/min/cm2
3. Measurements for gridded membranes will differ

The pores of microporous membrane filters act as small capillaries.  When hydrophilic membranes come into contact with water, capillary action associated with surface tension forces causes the water to spontaneously enter and fill the pores.  In this manner, the membranes are easily wetted and allow the bulk flow of water through the pores.  Once wetted, hydrophilic membranes will not allow the bulk flow of air or other gasses, unless they are applied at pressures greater than the membrane’s bubble point.

Hydrophilic membrane filters are typically used with water and aqueous solutions.  They can also be used with compatible non-aqueous fluids.  Hydrophilic membrane filters are typically not used for air, gas or vent filtration since the filters would block flow if inadvertently wetted, by condensation for example.

When hydrophobic membranes come into contact with water, surface tension forces act to repel the water from the pores.  Water will not enter the pores and the membranes will act as a barrier to water flow, unless the water is applied at pressures greater than the membrane’s water entry pressure.  Low surface tension fluids, such as alcohols, can spontaneously enter and fill the pores of hydrophobic membranes.  Once all the air in the pores is displaced, there are no longer any surface tension forces and water can easily enter the pores, displace the low surface tension fluid, and pass through the membrane.  The membrane will then allow bulk flow of water for as long as the pore remain water filled.  If the membrane is allowed to dry (i.e. air enters the pores), then it must be pre-wet with a low surface tension fluid again prior to use with water.

Hydrophobic membrane filters are typically used with compatible non-aqueous fluids.  They are also commonly used as air, gas, or vent filters.  Hydrophobic membrane filters are sometimes used with water or aqueous solutions; and, in these applications, they must first be prewet with a low surface tension, water miscible fluid prior to use.

Q. What is the difference between nominal and absolute pore size ratings?

Nominal pore size ratings are provided as a general indication of filter retention.  It is understood that some quantity of particles greater than, and equal to, the nominal pore size ratings will pass through the filters into the filtrate.  Some manufactures may associate nominal pore size ratings with percentage filtration efficiencies. Nominal pore size ratings vary from manufacturer to manufacturer and, consequently, are not necessarily equivalent. Filters from different manufactures with similar nominal pore size ratings may not actually exhibit similar retention characteristics.

Absolute pore size ratings are typically based on retention studies performed using challenge suspensions of standard microorganism cultures or particles of known size. Absolute pore size ratings represent the size of the smallest microorganisms or particles completely retained during these studies. Absolute pore size ratings are almost always correlated to bubble point specifications that are used for quality control during membrane manufacturing. For the most part, absolute pore size ratings, especially those based on microbial retention, are comparable from manufacturer to manufacturer. There is more uncertainty for absolute pore size ratings based on particle retention studies, especially for pore size ratings <0.2µm, since there are no standard methods for these studies.

Regardless of pore size ratings, it is important to understand that application conditions do influence particle retention. Even filters with absolute pore size ratings can be operated in conditions that will allow unexpectedly sized particles to pass.

Depth filters are constructed with relatively thick filtration media and typically have nominal pore size ratings >1µm. Due to their large void volume, they capture significant amounts of particulate within their pore structure.
Membrane filters are typically composed of polymers that have been chemically processed, resulting in highly porous thin films with microscopic pore structures. Membrane filters typically have absolute pore size ratings <1µm, with some exceptions. Because of their very fine pore structure, membrane filters tend to trap the majority of particles on the surface. However, smaller particles with diameters near or below the pore size rating can be captured within the membrane or pass through the membrane.

The pore size refers to the diameter of the individual pores in a membrane filter.   Pore size is typically specified in micrometers (µm).   Most membranes and filter media actually contain a distribution of pore sizes.  Nominal pore size ratings typically refer to the predominant pore size of a filtration media; pores larger and smaller than the nominal rating may be present.  Absolute pore size ratings typically refer to the largest pore size of a membrane and it is expected that all pores will be equal to or smaller than the absolute rating.

For the polycarbonate track-etch (PCTE) and polyester track-etch (PETE) membrane filters, porosity is the percent of the total surface area occupied by the pores; it typically ranges from <1% to 16%.  For the other membrane filters, porosity is the percent of the total volume occupied by the pores; it typically ranges from 40 to 80%.

You can find the Sterlitech compatibility guide posted at https://www.sterlitech.com/pub/media/pdf_resources/Chemical_Compatibility.pdf.  It is important to realize that application conditions, such
as operating temperature, affect compatibility.  Please contact us at sales@sterlitech.com if you need assistance.

To ensure ease of use, the membrane filters as stacked in their packaging are interleafed with layers of separator paper.  In most cases, the membrane filters will be white in color except for the track-etch membranes which are colorless and translucent.  In some special cases, the membranes will be dyed dark grey to black in appearance.  In all cases, the separator paper will be a different color than the membrane and is usually not white.  Please contact us at sales@sterlitech.com if you need assistance.

The bubble point is the minimum amount of pressure required to push air bubbles through the largest pore of a wet membrane.  The bubble point is inversely proportional to the pore diameter, as the pore diameter decreases the bubble point increases and vice versa.

Retention efficiency of membrane filters can be directly measured by challenging the filters with suspensions of standard microorganism cultures or particles of known size.  Unfortunately, such efficiency testing is necessarily destructive.  However, since retention characteristics are dependent on pore size, it is possible to correlate destructive challenge testing results to non-destructive membrane bubble point tests.  In this manner, the relationship between membrane pore size and membrane bubble point is empirically determined.  Typically, a minimum bubble point can be determined and specified for a particular pore size rating.  The bubble point specification is then used for quality control during membrane manufacture.  The bubble point can also be used by the consumer as a nondestructive test to verify membrane integrity before and/or after use.    

MCE membranes feature fast flow rates, a high protein binding capacity, and great thermal stability, making them a staple for many environmental and biological laboratories. Furthermore, they are available as presterilized, individually wrapped membranes, and can include a gridded pattern for quantifying microbial growth.

Unfortunately, in most instances, we are unable to supply the MCE membrane filters with custom diameters. Please inquire with your Sterlitech sales representative about alternatives.

Sterile mixed cellulose esters (MCE) membrane filters are used in vast quantities for microbiological studies across many industries and are manufactured in very high volumes to accommodate this demand.  Economies of scale and process automation allow the sterile MCE membrane filters to be offered at lower pack costs compared to non-sterile MCE membrane filters.  Non-sterile MCE filters are used much less frequently, necessitating less efficient, smaller volume manufacturing runs and packaging methods.  Consequently, the non-sterile MCE membrane filters have intrinsically greater manufacturing costs and must be offered at higher prices.

The grid dimensions are 3mm x 3mm. The average number of squares for a 47 mm disc filter is 185.